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验证数据展示

  • IF Staining of mouse stomach using CL488-60130

    IF Staining of mouse stomach using CL488-60130

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using CoraLite® Plus 488 Gastrokine 1 antibody (CL488-60130, Clone: 3C10E3 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse stomach using CL488-60130

    IF Staining of mouse stomach using CL488-60130

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using CoraLite® Plus 488 Gastrokine 1 antibody (CL488-60130, Clone: 3C10E3 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated Gastrokine 1 Monoclonal antibody

Gastrokine 1 Monoclonal Antibody for IF-P
Cat No. CL488-60130

产品说明书

CloneNo. 3C10E3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF-P

别名

GKN1, CA11, BRICD1, AMP-18, AMP18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse stomach tissue
For other applications, we recommend the unconjugated version of this antibody, 60130-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60130 targets Gastrokine 1 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Gastrokine 1 fusion protein Ag6091 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 gastrokine 1
别名 GKN1, CA11, BRICD1, AMP-18, AMP18
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC059778
基因名称 Gastrokine 1
Gene ID (NCBI) 56287
RRIDAB_2934425
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS71
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Gastrokine 1 antibody CL488-60130Download protocol
Standard Protocols
Click here to view our Standard Protocols