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验证数据展示

  • FC experiment of Balb/c mouse peritoneal macrophages using 98335-3-RR (same clone as 98335-3-PBS)

    FC experiment of Balb/c mouse peritoneal macrophages using 98335-3-RR (same clone as 98335-3-PBS)

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse Galectin-3 Rabbit RecAb (98335-3-RR, Clone:242453H5) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b. Cells were not fixed. This data was developed using the same antibody clone with 98335-3-PBS in a different storage buffer formulation.

  • FC experiment of Balb/c mouse peritoneal macrophages using 98335-3-RR (same clone as 98335-3-PBS)

    FC experiment of Balb/c mouse peritoneal macrophages using 98335-3-RR (same clone as 98335-3-PBS)

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse Galectin-3 Rabbit RecAb (98335-3-RR, Clone:242453H5)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98335-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98335-3-RR

    Affinity and Kinetic Characterization of 98335-3-RR

    Biolayer interferometry (BLl) kinetic assays of 98335-3-RR against Mouse Galectin-3 were performed. The affinity constant is 49.5 pM.

Anti-Mouse Galectin-3 Rabbit Recombinant Antibody, PBS Only

Galectin-3 Uni-rAb® Recombinant Antibody for FC
Cat No. 98335-3-PBS

产品说明书

CloneNo. 242453H5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Gal-3, Lgals3, 242453H5, 35 kDa lectin

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98335-3-PBS targets Galectin-3 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1232

Product name: Recombinant Mouse Galectin-3 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 1-264 aa of

Sequence: MADSFSLNDALAGSGNPNPQGYPGAWGNQPGAGGYPGAAYPGAYPGQAPPGAYPGQAPPGAYPGQAPPSAYPGPTAPGAYPGPTAPGAYPGQPAPGAFPGQPGAPGAYPQCSGGYPAAGPYGVPAGPLTVPYDLPLPGGVMPRMLITIMGTVKPNANRIVLDFRRGNDVAFHFNPRFNENNRRVIVCNTKQDNNWGKEERQSAFPFESGKPFKIQVLVEADHFKVAVNDAHLLQYNHRMKNLREISQLGISGDITLTSANHAMI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lectin, galactose binding, soluble 3
别名 Gal-3, Lgals3, 242453H5, 35 kDa lectin
计算分子量28 kDa
基因名称 Lgals3
Gene ID (NCBI) 16854
RRIDAB_3745985
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP16110
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

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