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验证数据展示

  • IF Staining of rat colon using CL594-11858

    IF Staining of rat colon using CL594-11858

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using CoraLite®594 Galectin-1 antibody (CL594-11858) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using CL594-11858

    IF Staining of HeLa using CL594-11858

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Galectin-1 antibody (CL594-11858) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Galectin-1 Polyclonal antibody

Galectin-1 Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL594-11858

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P

别名

Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1, Galaptin

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inrat colon tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LGALS1 Ready-To-Use IHC Kit. LGALS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11858-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-11858 targets Galectin-1 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2432

Product name: Recombinant human Galectin 1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC020675

Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lectin, galactoside-binding, soluble, 1
别名 Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1, Galaptin
计算分子量 15 kDa
GenBank蛋白编号BC020675
基因名称 Galectin-1
Gene ID (NCBI) 3956
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09382
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

实验方案

Product Specific Protocols
IF protocol for CL594 Galectin-1 antibody CL594-11858Download protocol
Standard Protocols
Click here to view our Standard Protocols
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