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验证数据展示

  • WB analysis of A375 using 51049-2-AP

    WB analysis of A375 using 51049-2-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 51049-2-AP

    IHC staining of human prostate cancer using 51049-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51049-2-AP (Galc antibody) at dilution of 1:50 (under 10x lens).

Galc Polyclonal antibody

Galc Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51049-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, human brain tissue, human placenta tissue, mouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51049-2-AP targets Galc in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Galc fusion protein Ag0684 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 galactosylceramidase
别名 2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher
计算分子量 77 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC086671
基因名称 Galc
Gene ID (NCBI) 14420
RRIDAB_670391
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54818
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALC, also named as Galactosylceramidase , belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is an enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. (PMID:8634707 )

实验方案

Product Specific Protocols
WB protocol for Galc antibody 51049-2-APDownload protocol
IHC protocol for Galc antibody 51049-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols