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验证数据展示

  • IF Staining of K-562 using CL488-18147

    IF Staining of K-562 using CL488-18147

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 GYPC antibody (CL488-18147) at dilution of 1:200.

CoraLite® Plus 488-conjugated GYPC Polyclonal antibody

GYPC Polyclonal Antibody for IF/ICC
Cat No. CL488-18147

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

CD236, CD236R, GE, GLPC, Glycoconnectin, Glycophorin C, Glycophorin D, Glycoprotein beta, GPC, GPD, GYPC, GYPD, PAS 2, Sialoglycoprotein D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 18147-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18147 targets GYPC in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 GYPC fusion protein Ag12825 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycophorin C (Gerbich blood group)
别名 CD236, CD236R, GE, GLPC, Glycoconnectin, Glycophorin C, Glycophorin D, Glycoprotein beta, GPC, GPD, GYPC, GYPD, PAS 2, Sialoglycoprotein D
计算分子量 128 aa, 14 kDa
观测分子量 37 kDa
GenBank蛋白编号BC106051
基因名称 GYPC
Gene ID (NCBI) 2995
RRIDAB_3672689
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04921
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GYPC antibody CL488-18147Download protocol
Standard Protocols
Click here to view our Standard Protocols