验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse lung tissue, rat lung tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | mouse brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
产品信息
19011-1-AP targets GUCY1B3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5196 Product name: Recombinant human GUCY1B3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 282-619 aa of BC047620 Sequence: EGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETKQDDD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanylate cyclase 1, soluble, beta 3 |
| 别名 | GCS-beta-1, GCS beta 3, GCS beta 1, GC SB3, GC S beta 1 |
| 计算分子量 | 71 kDa |
| 观测分子量 | 67-71 kDa |
| GenBank蛋白编号 | BC047620 |
| 基因名称 | GUCY1B3 |
| Gene ID (NCBI) | 2983 |
| RRID | AB_2115817 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q02153 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GUCY1B3 antibody 19011-1-AP | Download protocol |
| IHC protocol for GUCY1B3 antibody 19011-1-AP | Download protocol |
| IP protocol for GUCY1B3 antibody 19011-1-AP | Download protocol |
| WB protocol for GUCY1B3 antibody 19011-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO. | ||
J Ethnopharmacol Modified Zhenwu Decoction improved intestinal barrier function of experimental colitis through activation of sGC-mediated cGMP/PKG signaling | ||
Clin Sci (Lond) Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.
| ||
Sci Rep Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. | ||
Cell Tissue Res Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle. | ||
World Neurosurg Upregulation of Connexin 40 Mediated by Nitric Oxide Attenuates Cerebral Vasospasm After Subarachnoid Hemorrhage via the Nitric Oxide-Cyclic Guanosine Monophosphate-Protein Kinase G Pathway. |
