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验证数据展示

  • WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    IP experiment of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    IP result of anti-GTF3C4 (IP:17653-1-AP, 3ug; Detection:17653-1-AP 1:800) with HeLa cells lysate 875ug. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

    IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GTF3C4 antibody included
  • IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

    IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GTF3C4 antibody included
  • IF Staining of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    IF Staining of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17653-1-AP (GTF3C4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

GTF3C4 Polyclonal antibody, PBS Only

GTF3C4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17653-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GTF3C4 Ready-To-Use IHC Kit. GTF3C4 primary antibody included.

产品信息

17653-1-PBS targets GTF3C4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11898

Product name: Recombinant human GTF3C4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 473-822 aa of BC094774

Sequence: GSVRTHGIAVSPCGAYLAIITTEGMINGLHPVNKNYQVQFVTLKTFEEAAAQLLESSVQNLFKQVDLIDLVRWKILKDKHIPQFLQEALEKKIESSGVTYFWRFKLFLLRILYQSMQKTPSEALWKPTHEDSKILLVDSPGMGNADDEQQEEGTSSKQVVKQGLQERSKEGDVEEPTDDSLPTTGDAGGREPMEEKLLEIQGKIEAVEMHLTREHMKRVLGEVYLHTWITENTSIPTRGLCNFLMSDEEYDDRTARVLIGHISKKMNKQTFPEHCSLCKEILPFTDRKQAVCSNGHIWLRCFLTYQSCQSLIYRRCLLHDSIARHPAPEDPDWIKRLLQSPCPFCDSPVF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIIC, polypeptide 4, 90kDa
别名
计算分子量 822 aa, 92 kDa
观测分子量 92 kDa
GenBank蛋白编号BC094774
基因名称 GTF3C4
Gene ID (NCBI) 9329
RRIDAB_2115253
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKN8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GTF3C4, also named as TFIIIC90, is essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. It has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3.

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