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验证数据展示

  • WB analysis of HeLa using 17653-1-AP

    WB analysis of HeLa using 17653-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 17653-1-AP

    IP experiment of HeLa using 17653-1-AP

    IP result of anti-GTF3C4 (IP:17653-1-AP, 3ug; Detection:17653-1-AP 1:800) with HeLa cells lysate 875ug.

  • IHC staining of human breast cancer using 17653-1-AP

    IHC staining of human breast cancer using 17653-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF3C4 antibody included
  • IHC staining of human breast cancer using 17653-1-AP

    IHC staining of human breast cancer using 17653-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF3C4 antibody included
  • IF Staining of HeLa using 17653-1-AP

    IF Staining of HeLa using 17653-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17653-1-AP (GTF3C4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GTF3C4 Polyclonal antibody

GTF3C4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17653-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

GTF3C4, KAT12, TF3C delta, TFIII90, TFIIIC 90 kDa subunit, TFiiiC2 90, TFIIIC90, TFIIICdelta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GTF3C4 Ready-To-Use IHC Kit. GTF3C4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17653-1-AP targets GTF3C4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag11898

Product name: Recombinant human GTF3C4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 473-822 aa of BC094774

Sequence: GSVRTHGIAVSPCGAYLAIITTEGMINGLHPVNKNYQVQFVTLKTFEEAAAQLLESSVQNLFKQVDLIDLVRWKILKDKHIPQFLQEALEKKIESSGVTYFWRFKLFLLRILYQSMQKTPSEALWKPTHEDSKILLVDSPGMGNADDEQQEEGTSSKQVVKQGLQERSKEGDVEEPTDDSLPTTGDAGGREPMEEKLLEIQGKIEAVEMHLTREHMKRVLGEVYLHTWITENTSIPTRGLCNFLMSDEEYDDRTARVLIGHISKKMNKQTFPEHCSLCKEILPFTDRKQAVCSNGHIWLRCFLTYQSCQSLIYRRCLLHDSIARHPAPEDPDWIKRLLQSPCPFCDSPVF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIIC, polypeptide 4, 90kDa
别名 GTF3C4, KAT12, TF3C delta, TFIII90, TFIIIC 90 kDa subunit, TFiiiC2 90, TFIIIC90, TFIIICdelta
计算分子量 822 aa, 92 kDa
观测分子量 92 kDa
GenBank蛋白编号BC094774
基因名称 GTF3C4
Gene ID (NCBI) 9329
RRIDAB_2115253
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GTF3C4, also named as TFIIIC90, is essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. It has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3.

实验方案

Product Specific Protocols
WB protocol for GTF3C4 antibody 17653-1-APDownload protocol
IHC protocol for GTF3C4 antibody 17653-1-APDownload protocol
IF protocol for GTF3C4 antibody 17653-1-APDownload protocol
IP protocol for GTF3C4 antibody 17653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.

Authors - Ding Wang
View Article