验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, A375 cells, Jurkat cells |
| Positive IF/ICC detected in | A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
11596-1-AP targets TFIIE Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2136 Product name: Recombinant human TFIIE beta protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-291 aa of BC030572 Sequence: PSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | general transcription factor IIE, polypeptide 2, beta 34kDa |
| 别名 | GTF2E2, TFIIE-beta, TFIIE B, TF2E2 |
| 计算分子量 | 291 aa, 33 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC030572 |
| 基因名称 | GTF2E2 |
| Gene ID (NCBI) | 2961 |
| RRID | AB_2232722 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29084 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TFIIE Beta antibody 11596-1-AP | Download protocol |
| IF protocol for TFIIE Beta antibody 11596-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation. | ||
Cancer Gene Ther GTF2E2 downregulated by miR-340-5p inhibits the malignant progression of glioblastoma |
