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验证数据展示

  • IF Staining of mouse liver using CL488-67645

    IF Staining of mouse liver using CL488-67645

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 GSTT1 antibody (CL488-67645, Clone: 1A4C11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated GSTT1 Monoclonal antibody

GSTT1 Monoclonal Antibody for IF-P
Cat No. CL488-67645

产品说明书

CloneNo. 1A4C11

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse

应用

IF-P

别名

Glutathione transferase T1 1, GST class theta 1, GSTT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy GSTT1 Ready-To-Use IHC Kit. GSTT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67645-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67645 targets GSTT1 in IF-P applications and shows reactivity with Human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag8573

Product name: Recombinant human GSTT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-240 aa of BC007065

Sequence: MGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKGQHLSDACAQVNPLKKVPALKDGDFTLTESVAILLYLTRKYKVPDYWYPQDLQARARVDEYLAWQHTTLRRSCLRALWHKVMFPVFLGEPVSPQTLAATLAELDVTLQLLEDKFLQNKAFLTGPHISLADLVAITELMHPVGAGCQVFEGRPKLATWRQRVEAAVGEDLFQEAHEVILKAKDFPPADPTIKQKLMPWVLAMIR

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutathione S-transferase theta 1
别名 Glutathione transferase T1 1, GST class theta 1, GSTT1
计算分子量 240 aa, 27 kDa
观测分子量 28 kDa, 30 kDa
GenBank蛋白编号BC007065
基因名称 GSTT1
Gene ID (NCBI) 2952
RRIDAB_2934556
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30711
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GSTT1 antibody CL488-67645Download protocol
Standard Protocols
Click here to view our Standard Protocols