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验证数据展示

  • FC experiment of Jurkat using CL555-15902

    FC experiment of Jurkat using CL555-15902

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 555-conjugated GSTP1 Polyclonal antibody (CL555-15902)(red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 555-conjugated GSTP1 Polyclonal antibody

GSTP1 Polyclonal Antibody for FC (Intra)
Cat No. CL555-15902

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

EC:2.5.1.18, FAEES3, Glutathione S-transferase P, GST3, GST3/GST pi

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 555
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy GSTP1 Ready-To-Use IHC Kit. GSTP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15902-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-15902 targets GSTP1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8731

Product name: Recombinant human GSTP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of BC010915

Sequence: MPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYVSLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutathione S-transferase pi 1
别名 EC:2.5.1.18, FAEES3, Glutathione S-transferase P, GST3, GST3/GST pi
计算分子量 210 aa, 23 kDa
观测分子量 23-28 kDa
GenBank蛋白编号BC010915
基因名称 GSTP1
Gene ID (NCBI) 2950
RRIDAB_3084530
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09211
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutathione S-transferase P (GSTP1) conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 GSTP1 antibody CL555-15902Download protocol
Standard Protocols
Click here to view our Standard Protocols
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