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验证数据展示

  • IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)

    IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTK1 antibody included
  • WB analysis of HepG2 using 14535-1-AP (same clone as 14535-1-PBS)

    WB analysis of HepG2 using 14535-1-AP (same clone as 14535-1-PBS)

    WB result of GSTK1 antibody (14535-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)

    IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTK1 antibody included
  • IP experiment of Raji using 14535-1-AP (same clone as 14535-1-PBS)

    IP experiment of Raji using 14535-1-AP (same clone as 14535-1-PBS)

    IP result of anti-GSTK1 (IP:14535-1-AP, 3ug; Detection:14535-1-AP 1:1000) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

  • WB analysis using 14535-1-AP (same clone as 14535-1-PBS)

    WB analysis using 14535-1-AP (same clone as 14535-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GSTK1 Polyclonal antibody, PBS Only

GSTK1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14535-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSTK1 Ready-To-Use IHC Kit. GSTK1 primary antibody included.

产品信息

14535-1-PBS targets GSTK1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6036

Product name: Recombinant human GSTK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-226 aa of BC050715

Sequence: MGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutathione S-transferase kappa 1
别名
计算分子量 32 kDa
观测分子量 27 kDa
GenBank蛋白编号BC050715
基因名称 GSTK1
Gene ID (NCBI) 373156
RRIDAB_2115910
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2Q3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

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