• Featured Product
  • KD/KO验证

GSTK1 Polyclonal antibody

GSTK1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14535-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

DsbA-L, GST 13 13, GST, Glutathione S-transferase subunit 13, Glutathione S-transferase kappa 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, HUVEC cells, Raji cells, L02 cells, mouse testis tissue, rat testis tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman testis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GSTK1 Ready-To-Use IHC Kit. GSTK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14535-1-AP targets GSTK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 GSTK1 fusion protein Ag6036 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutathione S-transferase kappa 1
别名 DsbA-L, GST 13 13, GST, Glutathione S-transferase subunit 13, Glutathione S-transferase kappa 1
计算分子量 32 kDa
观测分子量 27 kDa
GenBank蛋白编号BC050715
基因名称 GSTK1
Gene ID (NCBI) 373156
RRIDAB_2115910
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2Q3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

实验方案

Product Specific Protocols
WB protocol for GSTK1 antibody 14535-1-APDownload protocol
IHC protocol for GSTK1 antibody 14535-1-APDownload protocol
IP protocol for GSTK1 antibody 14535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Proteome Res

Proteomic Analysis of Hearts from Akita Mice Suggests Increases in Soluble Epoxide Hydrolase and Antioxidative Programming are Key Changes in Early Stages of Diabetic Cardiomyopathy.

Authors - Dewey Shannamar S
mouseWB

J Gerontol A Biol Sci Med Sci

PMID: 24285747

Authors - Rojanathammanee Lalida L
mouseWB

Age (Dordr)

PMID: 25001375

Authors - Lalida Rojanathammanee
humanWB

Exp Mol Pathol

Aberrant expression of redox regulatory proteins in patients with concomitant primary Sclerosing cholangitis/inflammatory bowel disease.

Authors - Dennis R Petersen
humanCoIP

FASEB J

The role of chemerin in the regulation of cGAS-STING pathway in gestational diabetes mellitus placenta

Authors - Xuan Zhou
human,ratWB

Sci Total Environ

m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance

Authors - Jingyuan Zhang
Loading...