验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HUVEC cells, Raji cells, L02 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human testis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GSTK1 Ready-To-Use IHC Kit. GSTK1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14535-1-AP targets GSTK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GSTK1 fusion protein Ag6036 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutathione S-transferase kappa 1 |
| 别名 | DsbA-L, GST 13 13, GST, Glutathione S-transferase subunit 13, Glutathione S-transferase kappa 1 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC050715 |
| 基因名称 | GSTK1 |
| Gene ID (NCBI) | 373156 |
| RRID | AB_2115910 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2Q3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GSTK1 antibody 14535-1-AP | Download protocol |
| IHC protocol for GSTK1 antibody 14535-1-AP | Download protocol |
| IP protocol for GSTK1 antibody 14535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Proteome Res Proteomic Analysis of Hearts from Akita Mice Suggests Increases in Soluble Epoxide Hydrolase and Antioxidative Programming are Key Changes in Early Stages of Diabetic Cardiomyopathy. | ||
Exp Mol Pathol Aberrant expression of redox regulatory proteins in patients with concomitant primary Sclerosing cholangitis/inflammatory bowel disease. | ||
FASEB J The role of chemerin in the regulation of cGAS-STING pathway in gestational diabetes mellitus placenta | ||
Sci Total Environ m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance |
