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验证数据展示

  • WB analysis using 85954-2-RR (same clone as 85954-2-PBS)

    WB analysis using 85954-2-RR (same clone as 85954-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85954-2-RR (GSTA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85954-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 85954-2-RR (same clone as 85954-2-PBS)

    IF Staining of HEK-293 using 85954-2-RR (same clone as 85954-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GSTA4 antibody (85954-2-RR, Clone: 250352E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85954-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 85954-2-RR (same clone as 85954-2-PBS)

    IF Staining of HEK-293 using 85954-2-RR (same clone as 85954-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using GSTA4 antibody (85954-2-RR, Clone: 250352E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85954-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85954-2-RR

    Affinity and Kinetic Characterization of 85954-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85954-2-RR against Human GSTA4 were performed. The affinity constant is 0.489 nM.

GSTA4 Recombinant monoclonal antibody, PBS Only

GSTA4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85954-2-PBS

产品说明书

CloneNo. 250352E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

EC:2.5.1.18, Glutathione S transferase A4, Glutathione S transferase A4 4, Glutathione S-transferase A4, Glutathione S-transferase A4-4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85954-2-PBS targets GSTA4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11028

Product name: Recombinant human GSTA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-222 aa of BC015523

Sequence: MAARPKLHYPNGRGRMESVRWVLAAAGVEFDEEFLETKEQLYKLQDGNHLLFQQVPMVEIDGMKLVQTRSILHYIADKHNLFGKNLKERTLIDMYVEGTLDLLELLIMHPFLKPDDQQKEVVNMAQKAIIRYFPVFEKILRGHGQSFLVGNQLSLADVILLQTILALEEKIPNILSAFPFLQEYTVKLSNIPTIKRFLEPGSKKKPPPDEIYVRTVYNIFRP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutathione S-transferase alpha 4
别名 EC:2.5.1.18, Glutathione S transferase A4, Glutathione S transferase A4 4, Glutathione S-transferase A4, Glutathione S-transferase A4-4
计算分子量 222 aa, 27 kDa
观测分子量25 kDa
GenBank蛋白编号BC015523
基因名称 GSTA4
Gene ID (NCBI) 2941
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15217
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSTA4 (Glutathione S-Transferase Alpha 4) is an enzyme that plays a key role in detoxification and antioxidant defense. Its structure includes a GSH-binding site and a hydrophobic substrate-binding domain, functioning as a dimer. Dysregulation of GSTA4 is linked to diseases like cancer, neurodegeneration, and diabetes.

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