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验证数据展示

  • WB analysis using 85954-2-RR

    WB analysis using 85954-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85954-2-RR (GSTA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 85954-2-RR

    IF Staining of HEK-293 using 85954-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GSTA4 antibody (85954-2-RR, Clone: 250352E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HEK-293 using 85954-2-RR

    IF Staining of HEK-293 using 85954-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using GSTA4 antibody (85954-2-RR, Clone: 250352E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85954-2-RR

    Affinity and Kinetic Characterization of 85954-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85954-2-RR against Human GSTA4 were performed. The affinity constant is 0.489 nM.

GSTA4 Recombinant monoclonal antibody

GSTA4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85954-2-RR

产品说明书

CloneNo. 250352E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:2.5.1.18, Glutathione S transferase A4, Glutathione S transferase A4 4, Glutathione S-transferase A4, Glutathione S-transferase A4-4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HuH-7 cells, HEK-293T cells, fetal human brain tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85954-2-RR targets GSTA4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11028

Product name: Recombinant human GSTA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-222 aa of BC015523

Sequence: MAARPKLHYPNGRGRMESVRWVLAAAGVEFDEEFLETKEQLYKLQDGNHLLFQQVPMVEIDGMKLVQTRSILHYIADKHNLFGKNLKERTLIDMYVEGTLDLLELLIMHPFLKPDDQQKEVVNMAQKAIIRYFPVFEKILRGHGQSFLVGNQLSLADVILLQTILALEEKIPNILSAFPFLQEYTVKLSNIPTIKRFLEPGSKKKPPPDEIYVRTVYNIFRP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutathione S-transferase alpha 4
别名 EC:2.5.1.18, Glutathione S transferase A4, Glutathione S transferase A4 4, Glutathione S-transferase A4, Glutathione S-transferase A4-4
计算分子量 222 aa, 27 kDa
观测分子量25 kDa
GenBank蛋白编号BC015523
基因名称 GSTA4
Gene ID (NCBI) 2941
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15217
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTA4 (Glutathione S-Transferase Alpha 4) is an enzyme that plays a key role in detoxification and antioxidant defense. Its structure includes a GSH-binding site and a hydrophobic substrate-binding domain, functioning as a dimer. Dysregulation of GSTA4 is linked to diseases like cancer, neurodegeneration, and diabetes.

实验方案

Product Specific Protocols
IF protocol for GSTA4 antibody 85954-2-RRDownload protocol
WB protocol for GSTA4 antibody 85954-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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