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验证数据展示

  • WB analysis using 86508-3-RR (same clone as 86508-3-PBS)

    WB analysis using 86508-3-RR (same clone as 86508-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86508-3-RR (GSPT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86508-3-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 86508-3-RR (same clone as 86508-3-PBS)

    IF Staining of MCF-7 using 86508-3-RR (same clone as 86508-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using GSPT2 antibody (86508-3-RR, Clone: 251224C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86508-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86508-3-RR

    Affinity and Kinetic Characterization of 86508-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86508-3-RR against Human GSPT2 were performed. The affinity constant is 2.18 nM.

GSPT2 Recombinant monoclonal antibody, PBS Only

GSPT2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86508-3-PBS

产品说明书

CloneNo. 251224C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

EC:3.6.5.-, eRF3b, Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, G1 to S phase transition 2, GST2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSPT2 Ready-To-Use IHC Kit. GSPT2 primary antibody included.

产品信息

86508-3-PBS targets GSPT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3641

Product name: Recombinant human GSPT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC036077

Sequence: MDSGSSSSDSAPDCWDQVDMESTGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 G1 to S phase transition 2
别名 EC:3.6.5.-, eRF3b, Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, G1 to S phase transition 2, GST2
计算分子量 628 aa, 69 kDa
观测分子量 69-75 kDa
GenBank蛋白编号BC036077
基因名称 GSPT2
Gene ID (NCBI) 23708
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IYD1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSPT2, also named as eukaryotic peptide chain release factor GTP-binding subunit ERF3B or G1 to S phase transition protein 2 homolog, is a 628 amino acid protein, which belongs to the GTP-binding elongation factor family ERF3 subfamily. GSPT2 localizes in the cytoplasm and is highly expressed in IUCC stage II colorectal cancer (CRC). GSPT2 is involved in translation termination in response to the termination codons UAA, UAG, and UGA and may play a role as a potent stimulator of the release factor activity of ETF1. It may play a role in cell cycle progression.

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