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验证数据展示

  • WB analysis of U-251 using 17328-1-AP

    WB analysis of U-251 using 17328-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 17328-1-AP (GSG1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 17328-1-AP

    WB analysis using 17328-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17328-1-AP (GSG1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 17328-1-AP

    IHC staining of human tonsillitis using 17328-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17328-1-AP (GSG1L Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 17328-1-AP

    IHC staining of human tonsillitis using 17328-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17328-1-AP (GSG1L Antibody) at dilution of 1:50 (under 40x lens).

GSG1L Polyclonal antibody

GSG1L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17328-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GSG1 like, GSG1 like protein, GSG1L, PRO19651

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, U-251 cells, SGC-7901 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17328-1-AP targets GSG1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原

CatNo: Ag11307

Product name: Recombinant human GSG1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC016460

Sequence: MCLELFHSSNVIDGLKLNAFAAVFTVLSGLLGMVAHMMYTQVFQVTVSLGPEDWRPHSWDYGWSFCLAWGSFTCCMAASVTTLNSYTKTVIEFRHKRKVFEQGYREEPTFIDPEAIKYFRERMEKRDGSEEDFHLDCRHERYPARHQPHMADSWPRSSAQEAPELNRQCWVLGHWV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GSG1-like
别名 GSG1 like, GSG1 like protein, GSG1L, PRO19651
计算分子量 176 aa, 21 kDa
观测分子量 36-43 kDa
GenBank蛋白编号BC016460
基因名称 GSG1L
Gene ID (NCBI) 146395
RRIDAB_2878384
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXU4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSG1L is a component of the inner core of AMPAR complex. It modifies AMPA receptor (AMPAR) gating. GSG1L is mainly present on B lymphocytes. This antibody can recognize all the 4 isoforms of GSG1L. GSG1L was detected the isoform of 43 kDa in rat brain (PMID: 22813734).

实验方案

Product Specific Protocols
WB protocol for GSG1L antibody 17328-1-APDownload protocol
IHC protocol for GSG1L antibody 17328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
rat

Nat Commun

GSG1L-containing AMPA receptor complexes are defined by their spatiotemporal expression, native interactome and allosteric sites

Authors - Amanda M Perozzo
View Article