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验证数据展示

  • WB analysis of THP-1 using 30531-1-AP

    WB analysis of THP-1 using 30531-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30531-1-AP (GSDMD (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30531-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30531-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30531-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSDMD antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 30531-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30531-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30531-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSDMD antibody included

GSDMD Polyclonal antibody

GSDMD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30531-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD, Gasdermin-D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GSDMD Ready-To-Use IHC Kit. GSDMD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30531-1-AP targets GSDMD in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 GSDMD fusion protein Ag33627 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gasdermin D
别名 FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD, Gasdermin-D
计算分子量 484 aa, 53 kDa
观测分子量53 kDa, 43 kDa
GenBank蛋白编号BC008904
基因名称 GSDMD
Gene ID (NCBI) 79792
RRIDAB_3086353
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57764
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can detect 53 kDa full-length GSDMD and 43 kDa fragment as described in the literature (PMID: 28392147, PMID: 28979266).

实验方案

Product Specific Protocols
WB protocol for GSDMD antibody 30531-1-APDownload protocol
IHC protocol for GSDMD antibody 30531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols