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验证数据展示

  • WB analysis using 86451-3-RR (same clone as 86451-3-PBS)

    WB analysis using 86451-3-RR (same clone as 86451-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86451-3-RR (GRWD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86451-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86451-3-RR (same clone as 86451-3-PBS)

    IF Staining of HeLa using 86451-3-RR (same clone as 86451-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRWD1 antibody (86451-3-RR, Clone: 251273F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86451-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86451-3-RR (same clone as 86451-3-PBS)

    IF Staining of HepG2 using 86451-3-RR (same clone as 86451-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRWD1 antibody (86451-3-RR, Clone: 251273F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86451-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86451-3-RR

    Affinity and Kinetic Characterization of 86451-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86451-3-RR against Human GRWD1 were performed. The affinity constant is 0.203 nM.

GRWD1 Recombinant monoclonal antibody, PBS Only

GRWD1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86451-3-PBS

产品说明书

CloneNo. 251273F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Glutamate-rich WD repeat-containing protein 1, GRWD, KIAA1942, RRB1, WDR28

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86451-3-PBS targets GRWD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0367

Product name: Recombinant human GRWD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-236 aa of BC002440

Sequence: AESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSEGSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamate-rich WD repeat containing 1
别名 Glutamate-rich WD repeat-containing protein 1, GRWD, KIAA1942, RRB1, WDR28
计算分子量 49 kDa
观测分子量49-63 kDa
GenBank蛋白编号BC002440
基因名称 GRWD1
Gene ID (NCBI) 83743
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BQ67
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. (PMID:15885502)

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