验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, human brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
19956-1-AP targets mGluR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutamate receptor, metabotropic 2 |
| 别名 | GRM2, GLUR2, GPRC1B, mGlu2 |
| 计算分子量 | 96 kDa |
| 观测分子量 | 96 kDa, 120 kDa |
| GenBank蛋白编号 | NM_000839 |
| 基因名称 | mGluR2 |
| Gene ID (NCBI) | 2912 |
| RRID | AB_10638447 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14416 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRM2, also named GPRC1B and MGLUR2, belongs to the G-protein coupled receptor 3 families. GRM2 is a receptor for glutamate. The activity of GRM2 is mediated by a G-protein that inhibits adenylate cyclase activity. It may mediate the suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for mGluR2 antibody 19956-1-AP | Download protocol |
| WB protocol for mGluR2 antibody 19956-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Res Long-range epigenetic silencing associates with deregulation of Ikaros targets in colorectal cancer cells. | ||
Auton Neurosci Expression of group II metabotropic glutamate receptors in rat superior cervical ganglion | ||
Neurochem Int Red nucleus mGluR2 but not mGluR3 mediates inhibitory effect in the development of SNI-induced neuropathological pain by suppressing the expressions of TNF-α and IL-1β |
