验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, HeLa cells, rat brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IHC | See 2 publications below |
产品信息
19956-1-AP targets mGluR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glutamate receptor, metabotropic 2 |
别名 | GLUR2, GPRC1B, GRM2, mGlu2, mGluR2 |
计算分子量 | 96 kDa |
观测分子量 | 96 kDa, 120 kDa |
GenBank蛋白编号 | NM_000839 |
基因名称 | mGluR2 |
Gene ID (NCBI) | 2912 |
RRID | AB_10638447 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q14416 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRM2, also named as GPRC1B and MGLUR2, belongs to the G-protein coupled receptor 3 family. GRM2 is a receptor for glutamate. The activity of GRM2 is mediated by a G-protein that inhibits adenylate cyclase activity. It may mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
实验方案
Product Specific Protocols | |
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WB protocol for mGluR2 antibody 19956-1-AP | Download protocol |
IHC protocol for mGluR2 antibody 19956-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Res Long-range epigenetic silencing associates with deregulation of Ikaros targets in colorectal cancer cells. | ||
Auton Neurosci Expression of group II metabotropic glutamate receptors in rat superior cervical ganglion | ||
Neurochem Int Red nucleus mGluR2 but not mGluR3 mediates inhibitory effect in the development of SNI-induced neuropathological pain by suppressing the expressions of TNF-α and IL-1β |