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验证数据展示

  • WB analysis using 85942-1-RR (same clone as 85942-1-PBS)

    WB analysis using 85942-1-RR (same clone as 85942-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85942-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 85942-1-RR (same clone as 85942-1-PBS)

    IHC staining of mouse eye using 85942-1-RR (same clone as 85942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85942-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse eye using 85942-1-RR (same clone as 85942-1-PBS)

    IF Staining of mouse eye using 85942-1-RR (same clone as 85942-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using GRK1 antibody (85942-1-RR, Clone: 250433A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85942-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85942-1-RR

    Affinity and Kinetic Characterization of 85942-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85942-1-RR against Human GRK1 were performed. The affinity constant is 3.77 nM.

GRK1 Recombinant antibody, PBS Only

GRK1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85942-1-PBS

产品说明书

CloneNo. 250433A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85942-1-PBS targets GRK1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GRK1 fusion protein Ag18063 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 G protein-coupled receptor kinase 1
别名 EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1
计算分子量 563 aa, 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC140945
基因名称 GRK1
Gene ID (NCBI) 6011
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15835
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.