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验证数据展示

  • WB analysis of Y79 using 24606-1-AP (same clone as 24606-1-PBS)

    WB analysis of Y79 using 24606-1-AP (same clone as 24606-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 24606-1-AP (GRK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24606-1-PBS in a different storage buffer formulation.

  • IP experiment of Y79 using 24606-1-AP (same clone as 24606-1-PBS)

    IP experiment of Y79 using 24606-1-AP (same clone as 24606-1-PBS)

    IP result of anti-GRK1 (IP:24606-1-AP, 4ug; Detection:24606-1-AP 1:500) with Y79 cells lysate 1240ug. This data was developed using the same antibody clone with 24606-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 24606-1-AP (same clone as 24606-1-PBS)

    IHC staining of mouse eye using 24606-1-AP (same clone as 24606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24606-1-AP (GRK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24606-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse eye using 24606-1-AP (same clone as 24606-1-PBS)

    IF Staining of mouse eye using 24606-1-AP (same clone as 24606-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using GRK1 antibody (24606-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24606-1-PBS in a different storage buffer formulation.

GRK1 Polyclonal antibody, PBS Only

GRK1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 24606-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24606-1-PBS targets GRK1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18063

Product name: Recombinant human GRK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 339-563 aa of BC140945

Sequence: AVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor kinase 1
别名 EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1
计算分子量 563 aa, 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC140945
基因名称 GRK1
Gene ID (NCBI) 6011
RRIDAB_2879635
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15835
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.

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