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验证数据展示

  • WB analysis using 20851-1-AP

    WB analysis using 20851-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20851-1-AP (GRINA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20851-1-AP

    IHC staining of human placenta using 20851-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20851-1-AP (GRINA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 20851-1-AP

    IHC staining of human placenta using 20851-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20851-1-AP (GRINA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GRINA Polyclonal antibody

GRINA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20851-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Glutamate [NMDA] receptor-associated protein 1, HNRGW, LFG1, Protein lifeguard 1, Putative MAPK-activating protein PM02

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20851-1-AP targets GRINA in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14716

Product name: Recombinant human GRINA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-170 aa of BC041788

Sequence: MSHEKSFLVSGDNYPPPNPGYPGGPQPPMPPYAQPPYPGAPYPQPPFQPSPYGQPGYPHGPSPYPQGGYPQGPYPQGGYPQGPYPQEGYPQGPYPQGGYPQGPYPQNPFPPNPYGQPQVFPGQDPDSPQHGNYQEEGPPSYYDNQDFPATNWDDKSIRQAFIRKVFLVLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding)
别名 Glutamate [NMDA] receptor-associated protein 1, HNRGW, LFG1, Protein lifeguard 1, Putative MAPK-activating protein PM02
计算分子量 371 aa, 41 kDa
观测分子量49 kDa
GenBank蛋白编号BC041788
基因名称 GRINA
Gene ID (NCBI) 2907
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z429
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamate Receptor Ionotropic NMDA-Associated Protein 1 (GRINA, also known as TMBIM3) is a transmembrane protein involved in calcium homeostasis and cell survival. It plays a crucial role in regulating intracellular calcium levels, which are essential for processes such as cell survival, neurotransmitter release, and apoptosis (PMID: 32124700). GRINA also involves vesicle transport and sorting, particularly in secretory cells like neurons and mammary glands (PMID: 31426446). Additionally, GRINA has been implicated in regulating aerobic glycolysis and tumor progression in gastric cancer (PMID: 30541591).

实验方案

Product Specific Protocols
IHC protocol for GRINA antibody 20851-1-APDownload protocol
WB protocol for GRINA antibody 20851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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