CoraLite®594-conjugated GRIA1 Monoclonal antibody
GRIA1 Monoclonal Antibody for FC (Intra), IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Pig, Mouse, Rat, Rabbit
Applications
FC (Intra), IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1F4A1
验证数据展示
经过测试的应用
| Positive IF detected in | mouse brain tissue |
| Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67642-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67642 targets GRIA1 in IF, FC (Intra) applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.
| Tested Applications | FC (Intra), IF-P |
| Tested Reactivity | Human, Pig, Mouse, Rat, Rabbit |
| Immunogen | GRIA1 fusion protein Ag28932 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glutamate receptor, ionotropic, AMPA 1 |
| Synonyms | GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1 |
| Calculated Molecular Weight | 906 aa, 102 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC111734 |
| Gene Symbol | GRIA1 |
| Gene ID (NCBI) | 2890 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42261 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GRIA1 antibody CL594-67642 | Download protocol |
| FC protocol for CL594 GRIA1 antibody CL594-67642 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
