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GRIA1 Polyclonal antibody

GRIA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Rat, Mouse and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25012-1-AP

Print datasheet

Synonyms

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

验证数据展示

  • IHC staining of human brain using 25012-1-AP

    IHC staining of human brain using 25012-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25012-1-AP (GRIA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25012-1-AP

    IHC staining of human brain using 25012-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25012-1-AP (GRIA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 25012-1-AP

    WB analysis using 25012-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25012-1-AP (GRIA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 25012-1-AP

    WB analysis using 25012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25012-1-AP (GRIA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25012-1-AP

    IHC staining of mouse brain using 25012-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25012-1-AP (GRIA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25012-1-AP targets GRIA1 in WB, IHC, ELISA applications and shows reactivity with human, Rat, Mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, Rat, Mouse
Cited Reactivitymouse
Immunogen GRIA1 fusion protein Ag21502 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotropic, AMPA 1
Synonyms GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1
Calculated Molecular Weight 906 aa, 102 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC111734
Gene Symbol GRIA1
Gene ID (NCBI) 2890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42261
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.

实验方案

Product Specific Protocols
WB protocol for GRIA1 antibody 25012-1-APDownload protocol
IHC protocol for GRIA1 antibody 25012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling

Authors - Baomei Xia
View Article