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验证数据展示

  • IHC staining of human ovary tumor using 15298-1-AP

    IHC staining of human ovary tumor using 15298-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15298-1-AP (GRB14 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 15298-1-AP

    IHC staining of human ovary tumor using 15298-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15298-1-AP (GRB14 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of DU 145 using 15298-1-AP

    WB analysis of DU 145 using 15298-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 15298-1-AP (GRB14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

GRB14 Polyclonal antibody

GRB14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15298-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GRB14, GRB14 adapter protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15298-1-AP targets GRB14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag7554

Product name: Recombinant human GRB14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-540 aa of BC053559

Sequence: LQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFFLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGCSSQSISPMRSISENSLVAMDFSGQKSRVIENPTEALSVAVEEGLAWRKKGCLRLGTHGSPTASSQSSATNMAIHRSQPWFHHKISRDEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 14
别名 GRB14, GRB14 adapter protein
计算分子量 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC053559
基因名称 GRB14
Gene ID (NCBI) 2888
RRIDAB_2112892
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14449
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRB14 belongs to the GRB7/10/14 family. It is an adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. GRB14 is a target for a PDGF regulated serine kinase, an interaction that does not require PDGFR-GRB14 association. GRB14 is about 58-65kd in WB test.

实验方案

Product Specific Protocols
WB protocol for GRB14 antibody 15298-1-APDownload protocol
IHC protocol for GRB14 antibody 15298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biochim Biophys Acta Mol Basis Dis

Harnessing integrated bioinformatics to identify new diagnostic and therapeutic strategies for heart failure

Authors - Shuo Sun
View Article