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验证数据展示

  • WB analysis using 28872-1-AP

    WB analysis using 28872-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28872-1-AP (GRB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 28872-1-AP

    IHC staining of human liver using 28872-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28872-1-AP (GRB10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GRB10 Polyclonal antibody

GRB10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28872-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, RSS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HepG2 cells, mouse brain tissue, mouse pancreas tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28872-1-AP targets GRB10 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 10
别名 Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, RSS
计算分子量536aa,61 kDa; 594aa,67 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC024285
基因名称 GRB10
Gene ID (NCBI) 2887
RRIDAB_3086091
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRB10 is an adapter protein that modulates the coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain, and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation. GRB10 has 4 isoforms with the molecular mass of 60-70 kDa, which are produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for GRB10 antibody 28872-1-APDownload protocol
IHC protocol for GRB10 antibody 28872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols