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验证数据展示

  • IHC staining of human lymphoma using 14505-1-AP

    IHC staining of human lymphoma using 14505-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 14505-1-AP (GRAP antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lymphoma using 14505-1-AP

    IHC staining of human lymphoma using 14505-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 14505-1-AP (GRAP antibody) at dilution of 1:50 (under 40x lens).

GRAP Polyclonal antibody

GRAP Polyclonal Antibody for IHC, ELISA
Cat No. 14505-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA and More (1)

别名

GRAP, GRB2 related adapter protein, GRB2 related adaptor protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14505-1-AP targets GRAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag5962

Product name: Recombinant human GRAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-217 aa of BC063035

Sequence: MESVALYSFQATESDELAFNKGDTLKILNMEDDQNWYKAELRGVEGFIPKNYIRVKPHPWYSGRISRQLAEEILMKRNHLGAFLIRESESSPGEFSVSVNYGDQVQHFKVLREASGKYFLWEEKFNSLNELVDFYRTTTIAKKRQIFLRDEEPLLKSPGACFAQAQFDFSAQDPSQLSFRRGDIIEVLERPDPHWWRGRSCGRVGFFPRSYVQPVHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GRB2-related adaptor protein
别名 GRAP, GRB2 related adapter protein, GRB2 related adaptor protein
计算分子量 25 kDa
GenBank蛋白编号BC063035
基因名称 GRAP
Gene ID (NCBI) 10750
RRIDAB_2112704
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13588
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRAP antibody 14505-1-APDownload protocol
IHC protocol for GRAP antibody 14505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Life Sci

Grb2-related adaptor protein GRAP is a novel regulator of liver fibrosis

Authors - Xiaoyan Wu
View Article
mouseWB

Life Sci

Ablation of Brg1 in fibroblast/myofibroblast lineages attenuates renal fibrosis in mice with diabetic nephropathy

Authors - Xiaoyan Wu
View Article