×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86650-2-RR

    WB analysis using 86650-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86650-2-RR (GPX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 86650-2-RR

    IHC staining of mouse kidney using 86650-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86650-2-RR (GPX3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 86650-2-RR

    IF Staining of mouse kidney using 86650-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using GPX3 antibody (86650-2-RR, Clone: 251602E9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86650-2-RR

    Affinity and Kinetic Characterization of 86650-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86650-2-RR against Human GPX3 were performed. The affinity constant is below 1 pM.

GPX3 Recombinant monoclonal antibody

GPX3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86650-2-RR

产品说明书

CloneNo. 251602E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

EC:1.11.1.9, Glutathione peroxidase 3, GPx 3, GPx P, GPx-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86650-2-RR targets GPX3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5054

Product name: Recombinant human GPX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-72 aa of BC050378

Sequence: MARLLQASCLLSLLLAGFVSQSRGQEKSKMDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutathione peroxidase 3 (plasma)
别名 EC:1.11.1.9, Glutathione peroxidase 3, GPx 3, GPx P, GPx-3
计算分子量 226 aa, 26 kDa
观测分子量 20-27 kDa
GenBank蛋白编号BC050378
基因名称 GPX3
Gene ID (NCBI) 2878
RRIDAB_3745029
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22352
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).

实验方案

Product Specific Protocols
IF protocol for GPX3 antibody 86650-2-RRDownload protocol
IHC protocol for GPX3 antibody 86650-2-RRDownload protocol
WB protocol for GPX3 antibody 86650-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...