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验证数据展示

  • WB analysis using 86650-2-RR (same clone as 86650-2-PBS)

    WB analysis using 86650-2-RR (same clone as 86650-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86650-2-RR (GPX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86650-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 86650-2-RR (same clone as 86650-2-PBS)

    IHC staining of mouse kidney using 86650-2-RR (same clone as 86650-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86650-2-RR (GPX3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86650-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 86650-2-RR (same clone as 86650-2-PBS)

    IF Staining of mouse kidney using 86650-2-RR (same clone as 86650-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using GPX3 antibody (86650-2-RR, Clone: 251602E9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86650-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86650-2-RR

    Affinity and Kinetic Characterization of 86650-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86650-2-RR against Human GPX3 were performed. The affinity constant is below 1 pM.

GPX3 Recombinant monoclonal antibody, PBS Only

GPX3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86650-2-PBS

产品说明书

CloneNo. 251602E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

EC:1.11.1.9, Glutathione peroxidase 3, GPx 3, GPx P, GPx-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86650-2-PBS targets GPX3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5054

Product name: Recombinant human GPX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-72 aa of BC050378

Sequence: MARLLQASCLLSLLLAGFVSQSRGQEKSKMDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutathione peroxidase 3 (plasma)
别名 EC:1.11.1.9, Glutathione peroxidase 3, GPx 3, GPx P, GPx-3
计算分子量 226 aa, 26 kDa
观测分子量 20-27 kDa
GenBank蛋白编号BC050378
基因名称 GPX3
Gene ID (NCBI) 2878
RRIDAB_3745029
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22352
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).

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