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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GPT2 Polyclonal antibody

GPT2 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-16757

Print datasheet

Synonyms

AAT2, Alanine aminotransferase 2, ALT2, GPT 2, GPT2

验证数据展示

  • IF Staining of HepG2 using CL488-16757

    IF Staining of HepG2 using CL488-16757

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GPT2 antibody (CL488-16757) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-16757

    FC experiment of HepG2 using CL488-16757

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human GPT2 (CL488-16757) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16757-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16757 targets GPT2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen GPT2 fusion protein Ag10273 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamic pyruvate transaminase (alanine aminotransferase) 2
Synonyms AAT2, Alanine aminotransferase 2, ALT2, GPT 2, GPT2
Calculated Molecular Weight 523 aa, 58 kDa
Observed Molecular Weight 58 kDa, 47 kDa
GenBank Accession NumberBC062555
Gene Symbol GPT2
Gene ID (NCBI) 84706
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD30
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GPT2 antibody CL488-16757Download protocol
FC protocol for CL Plus 488 GPT2 antibody CL488-16757Download protocol
Standard Protocols
Click here to view our Standard Protocols