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验证数据展示

  • IF Staining of HepG2 using 67531-1-Ig (same clone as 67531-1-PBS)

    IF Staining of HepG2 using 67531-1-Ig (same clone as 67531-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • WB analysis using 67531-1-Ig (same clone as 67531-1-PBS)

    WB analysis using 67531-1-Ig (same clone as 67531-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse liver using 67531-1-Ig (same clone as 67531-1-PBS)

    WB analysis of mouse liver using 67531-1-Ig (same clone as 67531-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

GPT/ALT1 Monoclonal antibody, PBS Only

GPT/ALT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67531-1-PBS

产品说明书

CloneNo. 5F6B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, Indirect ELISA

别名

ALAT1, GPT, GPT/ ALT1, AAT1, Alanine aminotransferase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67531-1-PBS targets GPT/ALT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag10453

Product name: Recombinant human GPT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 147-496 aa of BC018207

Sequence: IPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAKFTLEYS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamic-pyruvate transaminase (alanine aminotransferase)
别名 ALAT1, GPT, GPT/ ALT1, AAT1, Alanine aminotransferase 1
计算分子量 496 aa, 55 kDa
观测分子量 52 kDa
GenBank蛋白编号BC018207
基因名称 ALT1
Gene ID (NCBI) 2875
RRIDAB_2882750
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP24298
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

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