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验证数据展示

  • WB analysis of HeLa using 20020-1-AP

    WB analysis of HeLa using 20020-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20020-1-AP

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 20020-1-AP

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 40x lens).

GPRASP1-Specific Polyclonal antibody

GPRASP1-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20020-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20020-1-AP targets GPRASP1-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor associated sorting protein 1
别名 GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443
计算分子量 157 kDa
观测分子量 157 kDa
GenBank蛋白编号NM_014710
基因名称 GPRASP1
Gene ID (NCBI) 9737
RRIDAB_10694144
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JY77
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPRASP1, also named as GASP, GASP1 and KIAA0443, belongs to the GPRASP family. GPRASP1 may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It is targets receptors for degradation in lysosomes. The antibody is specific to GPRASP1.

实验方案

Product Specific Protocols
WB protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
IHC protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Aging (Albany NY)

Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation

Authors - Baining Zhang
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