验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human brain tissue, mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
20020-1-AP targets GPRASP1-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor associated sorting protein 1 |
| 别名 | GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443 |
| 计算分子量 | 157 kDa |
| 观测分子量 | 157 kDa |
| GenBank蛋白编号 | NM_014710 |
| 基因名称 | GPRASP1 |
| Gene ID (NCBI) | 9737 |
| RRID | AB_10694144 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5JY77 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPRASP1, also named as GASP, GASP1 and KIAA0443, belongs to the GPRASP family. GPRASP1 may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It is targets receptors for degradation in lysosomes. The antibody is specific to GPRASP1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPRASP1-Specific antibody 20020-1-AP | Download protocol |
| WB protocol for GPRASP1-Specific antibody 20020-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation | ||
J Adv Res Development and characterization of endolysosomal trafficking targeting chimera degraders of α1A-adrenergic receptor |
