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验证数据展示

  • WB analysis of HeLa using 20020-1-AP

    WB analysis of HeLa using 20020-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20020-1-AP

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 20020-1-AP

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 40x lens).

GPRASP1-Specific Polyclonal antibody

GPRASP1-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20020-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20020-1-AP targets GPRASP1-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor associated sorting protein 1
别名 GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443
计算分子量 157 kDa
观测分子量 157 kDa
GenBank蛋白编号NM_014710
基因名称 GPRASP1
Gene ID (NCBI) 9737
RRIDAB_10694144
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JY77
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPRASP1, also named as GASP, GASP1 and KIAA0443, belongs to the GPRASP family. GPRASP1 may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It is targets receptors for degradation in lysosomes. The antibody is specific to GPRASP1.

实验方案

Product Specific Protocols
WB protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
IHC protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Aging (Albany NY)

Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation

Authors - Baining Zhang
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