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验证数据展示

  • IHC staining of mouse spleen using 20306-1-AP (same clone as 20306-1-PBS)

    IHC staining of mouse spleen using 20306-1-AP (same clone as 20306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20306-1-AP (GPR65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

  • FC experiment of RAW 264.7 using 20306-1-AP (same clone as 20306-1-PBS)

    FC experiment of RAW 264.7 using 20306-1-AP (same clone as 20306-1-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.5 ug GPR65 Polyclonal antibody (20306-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 20306-1-AP (same clone as 20306-1-PBS)

    IHC staining of human tonsillitis using 20306-1-AP (same clone as 20306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 20306-1-AP (same clone as 20306-1-PBS)

    IHC staining of human tonsillitis using 20306-1-AP (same clone as 20306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 20306-1-AP (same clone as 20306-1-PBS)

    IHC staining of mouse spleen using 20306-1-AP (same clone as 20306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20306-1-AP (GPR65 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

GPR65 Polyclonal antibody, PBS Only

GPR65 Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 20306-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, Indirect ELISA

别名

G protein coupled receptor 65, G-protein coupled receptor 65, hTDAG8, Psychosine receptor, T-cell death-associated gene 8 protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20306-1-PBS targets GPR65 in IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14139

Product name: Recombinant human GPR65 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-337 aa of BC035633

Sequence: CFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTSLNCVADPILYCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 65
别名 G protein coupled receptor 65, G-protein coupled receptor 65, hTDAG8, Psychosine receptor, T-cell death-associated gene 8 protein
计算分子量 337 aa, 39 kDa
GenBank蛋白编号BC035633
基因名称 GPR65
Gene ID (NCBI) 8477
RRIDAB_2878668
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYL9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).

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