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验证数据展示

  • WB analysis using 29934-1-AP (same clone as 29934-1-PBS)

    WB analysis using 29934-1-AP (same clone as 29934-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 29934-1-AP (GPR179 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29934-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 29934-1-AP (same clone as 29934-1-PBS)

    IHC staining of mouse liver using 29934-1-AP (same clone as 29934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29934-1-AP (GPR179 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29934-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 29934-1-AP (same clone as 29934-1-PBS)

    IHC staining of mouse liver using 29934-1-AP (same clone as 29934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29934-1-AP (GPR179 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29934-1-PBS in a different storage buffer formulation.

GPR179 Polyclonal antibody, PBS Only

GPR179 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29934-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

GPR158L1, G protein coupled receptor 179

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29934-1-PBS targets GPR179 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32180

Product name: Recombinant human GPR179 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-381 aa of NM_001004334.3

Sequence: LGGPRPIRSLPPLSSQVKPGSVPMQVPLEGAEAALAYLYSGDAQQLSQVNCSERYEARGAGAMPGLPPSLQGAAGTLAQAANFLNMLLQANDIRESSVEEDVEWYQALVRSVAEGDPRVYRALLTFNPPPGASHLQLALQATRTGEETILQDLSGNWVQEENPPGDLDTPALKKRVLTNDLGSLGSPKWPQADGYVGDTQQVRLSPPFLECQEGRLRPGWLITLSATFYGLKPDLSPEVRGQVQMDVDLQSVDINQCASGPGWYSNTHLCDLNSTQCVPLESQGFVLGRYLCRCRPGFYGASPSGGLEESDFQTTGQFGFPEGRSGRLLQCLPCPEGCTSCMDATPCLVEEAAVLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 179
别名 GPR158L1, G protein coupled receptor 179
计算分子量257 kDa
观测分子量260-270 kDa
GenBank蛋白编号NM_001004334.3
基因名称 GPR179
Gene ID (NCBI) 440435
RRIDAB_2923622
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PRD1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPR179 (Probable G-protein coupled receptor 179) belongs to the glutamate receptor subfamily of GPCRs, which is involved in signal transduction through retinal depolarizing bipolar cells. The protein has an EGF-like calcium binding domain and a seven transmembrane domain in the N-terminal region of the protein. Mutations in this gene are associated with congenital stationary night blindness type 1E. GPR179 is expressed in retinal bipolar cells and upregulated in rd1 mice retina with Pde6b mutations (PubMed: 22325361, PubMed: 22325362).

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