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GPR179 Polyclonal antibody

GPR179 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29934-1-AP

Print datasheet

Synonyms

G protein coupled receptor 179, GPR158L1, GPR179

验证数据展示

  • WB analysis using 29934-1-AP

    WB analysis using 29934-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 29934-1-AP (GPR179 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 29934-1-AP

    IHC staining of mouse liver using 29934-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29934-1-AP (GPR179 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 29934-1-AP

    IHC staining of mouse liver using 29934-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29934-1-AP (GPR179 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 29934-1-AP

    IF Staining of mouse eye using 29934-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using GPR179 antibody (29934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 29934-1-AP

    IF Staining of mouse eye using 29934-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using GPR179 antibody (29934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29934-1-AP targets GPR179 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen GPR179 fusion protein Ag32180 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 179
Synonyms G protein coupled receptor 179, GPR158L1, GPR179
Calculated Molecular Weight257 kDa
Observed Molecular Weight260-270 kDa
GenBank Accession NumberNM_001004334.3
Gene Symbol GPR179
Gene ID (NCBI) 440435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PRD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR179 (Probable G-protein coupled receptor 179) belongs to the glutamate receptor subfamily of GPCRs, which is involved in signal transduction through retinal depolarizing bipolar cells. The protein has an EGF-like calcium binding domain and a seven transmembrane domain in the N-terminal region of the protein. Mutations in this gene are associated with congenital stationary night blindness type 1E. GPR179 is expressed in retinal bipolar cells and upregulated in rd1 mice retina with Pde6b mutations (PubMed: 22325361, PubMed: 22325362).

实验方案

Product Specific Protocols
WB protocol for GPR179 antibody 29934-1-APDownload protocol
IHC protocol for GPR179 antibody 29934-1-APDownload protocol
IF protocol for GPR179 antibody 29934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols