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验证数据展示

  • IHC staining of mouse brain using 20213-1-AP

    IHC staining of mouse brain using 20213-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20213-1-AP (GPR150-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 20213-1-AP

    IF Staining of SH-SY5Y using 20213-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GPR150-Specific antibody (20213-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

GPR150-Specific Polyclonal antibody

GPR150-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20213-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

GPR150, Probable G-protein coupled receptor 150, PGR11, G protein coupled receptor 150

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20213-1-AP targets GPR150-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 150
别名 GPR150, Probable G-protein coupled receptor 150, PGR11, G protein coupled receptor 150
计算分子量 46 kDa
GenBank蛋白编号NM_199243
基因名称 GPR150
Gene ID (NCBI) 285601
RRIDAB_3669330
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NGU9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR150 belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR150.

实验方案

Product Specific Protocols
IF protocol for GPR150-Specific antibody 20213-1-APDownload protocol
IHC protocol for GPR150-Specific antibody 20213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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