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GPR146 Polyclonal antibody

GPR146 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 16334-1-AP

Print datasheet

Synonyms

G protein coupled receptor 146, GPR146, PGR8

验证数据展示

  • IHC staining of mouse liver using 16334-1-AP

    IHC staining of mouse liver using 16334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16334-1-AP (GPR146 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 16334-1-AP

    IHC staining of mouse kidney using 16334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16334-1-AP (GPR146 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16334-1-AP targets GPR146 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA
Tested Reactivity human, mouse
Immunogen GPR146 fusion protein Ag9456 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 146
Synonyms G protein coupled receptor 146, GPR146, PGR8
Calculated Molecular Weight 333 aa, 37 kDa
GenBank Accession NumberBC014241
Gene Symbol GPR146
Gene ID (NCBI) 115330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CH1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

As a member of GPCRs, GPR146 (G protein-coupled receptor 146) was initially discovered as a c-peptide signal body. GPR146 may act as a c peptide receptor to interact with proinsulin c-peptide to regulate insulin levels (PMID: 37926274). Mechanically, GPR146 induces hepatic sterol regulatory element binding protein 2 (SREBP2) via the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) signaling, consequently resulting in hepatic low-density lipoprotein (VLDL) secretion (PMID: 32491159).

实验方案

Product Specific Protocols
IHC protocol for GPR146 antibody 16334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols