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GPR142 Polyclonal antibody

GPR142 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 18820-1-AP

Print datasheet

Synonyms

G protein coupled receptor 142, GPR142, PGR2

验证数据展示

  • WB analysis of SH-SY5Y using 18820-1-AP

    WB analysis of SH-SY5Y using 18820-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18820-1-AP (GPR142 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 18820-1-AP

    FC experiment of SH-SY5Y using 18820-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug GPR142 antibody (18820-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18820-1-AP targets GPR142 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 142
Synonyms G protein coupled receptor 142, GPR142, PGR2
Calculated Molecular Weight 462 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC152987
Gene Symbol GPR142
Gene ID (NCBI) 350383
RRIDAB_10597384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z601
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR142, also named as PGR2, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody recognizes the 51-kDa GRP142.

实验方案

Product Specific Protocols
WB protocol for GPR142 antibody 18820-1-APDownload protocol
FC protocol for GPR142 antibody 18820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

BMC Genomics

The fiber diameter traits of Tibetan cashmere goats are governed by the inherent differences in stress, hypoxic, and metabolic adaptations: an integrative study of proteome and transcriptome.

Authors - Bingru Zhao
View Article