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验证数据展示

  • WB analysis using 16496-1-AP

    WB analysis using 16496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16496-1-AP (GPR137B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16496-1-AP

    IHC staining of human kidney using 16496-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16496-1-AP (GPR137B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16496-1-AP

    IHC staining of human kidney using 16496-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16496-1-AP (GPR137B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR137B Polyclonal antibody

GPR137B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16496-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

TM7SF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, RT-4 cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16496-1-AP targets GPR137B in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag9633

Product name: Recombinant human GPR137B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 253-399 aa of BC012100

Sequence: YNLFILSFSQNKSVHSFDYDWYNVSDQADLKNQLGDAGYVLFGVVLFVWELLPTTLVVYFFRVRNPTKDLTNPGMVPSHGFSPRSYFFDNPRRYDSDDDLAWNIAPQGLQGGFAPDYYDWGQQTNSFLAQAGTLQDSTLDPDKPSLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 137B
别名 TM7SF1
计算分子量 399 aa, 46 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC012100
基因名称 GPR137B
Gene ID (NCBI) 7107
RRIDAB_2247856
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60478
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR137B antibody 16496-1-APDownload protocol
IHC protocol for GPR137B antibody 16496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Bone

A role for G protein-coupled receptor 137b in bone remodeling in mouse and zebrafish.

Authors - K Urso
View Article
mouseWB

Biochem Biophys Res Commun

TM7SF1, an important autophagy regulatory protein in mouse podocytes.

Authors - Yunfei Hu
View Article