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GPR137B Polyclonal antibody

GPR137B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16496-1-AP

Print datasheet

Synonyms

GPR137B, TM7SF1

验证数据展示

  • WB analysis of human heart using 16496-1-AP

    WB analysis of human heart using 16496-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16496-1-AP (GPR137B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16496-1-AP

    IHC staining of human kidney using 16496-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16496-1-AP (GPR137B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16496-1-AP

    IHC staining of human kidney using 16496-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16496-1-AP (GPR137B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:100-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16496-1-AP targets GPR137B in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen GPR137B fusion protein Ag9633 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 137B
Synonyms GPR137B, TM7SF1
Calculated Molecular Weight 399 aa, 46 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC012100
Gene Symbol GPR137B
Gene ID (NCBI) 7107
RRIDAB_2247856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60478
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR137B antibody 16496-1-APDownload protocol
IHC protocol for GPR137B antibody 16496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Bone

A role for G protein-coupled receptor 137b in bone remodeling in mouse and zebrafish.

Authors - K Urso
View Article
mouseWB

Biochem Biophys Res Commun

TM7SF1, an important autophagy regulatory protein in mouse podocytes.

Authors - Yunfei Hu
View Article