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验证数据展示

  • WB analysis of HEK-293 using 20074-1-AP

    WB analysis of HEK-293 using 20074-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 20074-1-AP

    FC experiment of HEK-293 using 20074-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human GPR101-Specific (20074-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse kidney using 20074-1-AP

    IHC staining of mouse kidney using 20074-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20074-1-AP (GPR101-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 20074-1-AP

    IHC staining of mouse kidney using 20074-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20074-1-AP (GPR101-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR101-Specific Polyclonal antibody

GPR101-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 20074-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey

应用

WB, IHC, FC (Intra), ELISA

别名

GPR101, Probable G-protein coupled receptor 101

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, COS-7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20074-1-AP targets GPR101-Specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, monkey samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, monkey
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 101
别名 GPR101, Probable G-protein coupled receptor 101
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号NM_054021
基因名称 GPR101
Gene ID (NCBI) 83550
RRIDAB_10665356
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96P66
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR101, also named as GPCR6, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR101.

实验方案

Product Specific Protocols
WB protocol for GPR101-Specific antibody 20074-1-APDownload protocol
IHC protocol for GPR101-Specific antibody 20074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols