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验证数据展示

  • WB analysis of HEK-293 using 20074-1-AP

    WB analysis of HEK-293 using 20074-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 20074-1-AP

    FC experiment of HEK-293 using 20074-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human GPR101-Specific (20074-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GPR101-Specific Polyclonal antibody

GPR101-Specific Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 20074-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey

应用

WB, FC (Intra), ELISA

别名

Probable G-protein coupled receptor 101, GPR101

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, COS-7 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20074-1-AP targets GPR101-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human, monkey samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, monkey
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 101
别名 Probable G-protein coupled receptor 101, GPR101
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号NM_054021
基因名称 GPR101
Gene ID (NCBI) 83550
RRIDAB_10665356
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96P66
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR101, also named as GPCR6, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR101.

实验方案

Product Specific Protocols
WB protocol for GPR101-Specific antibody 20074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols