验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, 37°C incubated mouse brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26122-1-AP targets GPM6B in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag23507 Product name: Recombinant human GPM6B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-62 aa of BC047295 Sequence: MKPAMETAAEENTEQSQERKVNSRAEMEIGRYHWMYPGSKNHQYHPVPTLGDRASPLSSPGC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycoprotein M6B |
| 别名 | glycoprotein M6B, M6B |
| 观测分子量 | 27-36 kDa |
| GenBank蛋白编号 | BC047295 |
| 基因名称 | GPM6B |
| Gene ID (NCBI) | 2824 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13491 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPM6B (Glycoprotein M6B) is a highly conserved, multi-pass transmembrane glycoprotein encoded by the GPM6B gene on human chromosome Xp22.2. As a member of the proteolipid protein family, it is predominantly expressed in the central nervous system (in neurons and oligodendrocytes) and is involved in key processes such as myelination, regulation of serotonin transporter (SERT) trafficking, and actin cytoskeleton maintenance. Beyond neural functions, GPM6B also promotes bone formation by regulating osteoblast mineralization and influences smooth muscle cell differentiation via the TGF‑beta‑Smad2/3 pathway. Subcellularly, it localizes to the plasma membrane and lipid rafts. Genetic variations in GPM6B have been associated with several disorders, including Pelizaeus‑Merzbacher disease, major depressive disorder, and osteoarthritis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GPM6B antibody 26122-1-AP | Download protocol |
| IHC protocol for GPM6B antibody 26122-1-AP | Download protocol |
| IP protocol for GPM6B antibody 26122-1-AP | Download protocol |
| WB protocol for GPM6B antibody 26122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
