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验证数据展示

  • IHC staining of mouse brain using Biotin-15044

    IHC staining of mouse brain using Biotin-15044

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-15044 (GPM6A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Biotin Plus-conjugated GPM6A Polyclonal antibody

GPM6A Polyclonal Antibody for IHC
Cat No. Biotin-15044

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC

别名

Neuronal membrane glycoprotein M6-a, M6A, GPM6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin Plus
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-15044 targets GPM6A in IHC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC Application Description
经测试反应性 human, mouse, rat
免疫原 GPM6A fusion protein Ag6915 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycoprotein M6A
别名 Neuronal membrane glycoprotein M6-a, M6A, GPM6
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC022528
基因名称 GPM6A
Gene ID (NCBI) 2823
偶联类型 Biotin Plus
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51674
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycoprotein M6A (GPM6A, or M6A) is known as a multi-pass transmembrane protein and an abundant cell surface protein on neurons in the central nervous system (CNS). M6A belongs to the myelin proteolipid protein family and is involved in neuronal differentiation, including differentiation and migration of neuronal stem cell. M6A is identified as a retinal membrane protein that is strongly expressed at embryonic stages. In mouse adult retina, M6A plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation via regulation of MAPK and Src signaling pathways.

实验方案

Product Specific Protocols
IHC protocol for Biotin Plus GPM6A antibody Biotin-15044Download protocol
Standard Protocols
Click here to view our Standard Protocols