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验证数据展示

  • FC experiment of A549 using 98942-1-RR

    FC experiment of A549 using 98942-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human GPI Rabbit RecAb (98942-1-RR, Clone: 261335A1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IHC staining of human tonsil using 98942-1-RR

    IHC staining of human tonsil using 98942-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 98942-1-RR (GPI antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsil using 98942-1-RR

    IHC staining of human tonsil using 98942-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 98942-1-RR (GPI antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Anti-Human GPI Rabbit Recombinant Antibody

GPI Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98942-1-RR

产品说明书

CloneNo. 261335A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra)

别名

AMF, EC:5.3.1.9, Glucose-6-phosphate isomerase, GNPI, Neuroleukin

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98942-1-RR targets GPI in IHC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra) Application Description
经测试反应性 human
免疫原

unknow

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glucose phosphate isomerase
别名 AMF, EC:5.3.1.9, Glucose-6-phosphate isomerase, GNPI, Neuroleukin
基因名称 GPI
Gene ID (NCBI) 2821
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

GPI (Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase (PGI), Neuroleukinis (NLK), phosphohexose isomerase (PHI) or sperm antigen 36 (SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112).

实验方案

Product Specific Protocols
FC protocol for GPI antibody 98942-1-RRDownload protocol
IHC protocol for GPI antibody 98942-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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