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验证数据展示

  • WB analysis using 67178-1-Ig (same clone as 67178-1-PBS)

    WB analysis using 67178-1-Ig (same clone as 67178-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67178-1-Ig (GPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

    WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

    WB result of GPI antibody (67178-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 67178-1-Ig (same clone as 67178-1-PBS)

    IF Staining of PC-3 using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GPI antibody (67178-1-Ig, Clone: 2F11E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GPI Monoclonal antibody, PBS Only

GPI Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67178-1-PBS

产品说明书

CloneNo. 2F11E4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9, AMF

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67178-1-PBS targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7419

Product name: Recombinant human GPI protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-272 aa of BC004982

Sequence: MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glucose phosphate isomerase
别名 Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9, AMF
计算分子量 63 kDa
观测分子量 55-64 kDa
GenBank蛋白编号BC004982
基因名称 GPI
Gene ID (NCBI) 2821
RRIDAB_2882474
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP06744
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).

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