验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human skeletal muscle tissue, human brain tissue, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
产品信息
17263-1-AP targets GPD1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10977 Product name: Recombinant human GPD1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC028726 Sequence: MAAAPLKVCIVGSGNWGSAVAKIIGNNVKKLQKFASTVKMWVFEETVNGRKLTDIINNDHENVKYLPGHKLPENVVAMSNLSEAVQDADLLVFVIPHQFIHRICDEITGRVPKKALGITLIKGIDEGPEGLKLISDIIREKMGIDISVLMGANIANEVAAEKFCETTIGSKVMENGLLFKELLQTPNFRITVVDDADTVELCGALKNIVAVGAGFCDGLRCGDNTKAAVIRLGLMEMIAFARIFCKGQVSTATFLESCGVADLITTCYGGRNRRVAEAFARTGKTIEELEKEMLNGQKLQGPQTSAEVYRILKQKGLLDKFPLFTAVYQICYESRPVQEMLSCLQSHPEHT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycerol-3-phosphate dehydrogenase 1-like |
| 别名 | GPD1-L, GPD1 L, Glycerol-3-phosphate dehydrogenase 1-like protein, EC:1.1.1.8 |
| 计算分子量 | 351 aa, 38 kDa |
| 观测分子量 | 38-40 kDa |
| GenBank蛋白编号 | BC028726 |
| 基因名称 | GPD1L |
| Gene ID (NCBI) | 23171 |
| RRID | AB_2112359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N335 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycerol-3-phosphate dehydrogenase 1-like (GPD1L) is encoded by the gene GPD1L, which is located on chromosome 3p22.3. GPD1L is responsible for catalyzing the conversion of sn-glycerol 3-phosphate to glycerone phosphate. GPD1L displayed a dysregulated state in a wide variety of cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPD1L antibody 17263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD+ to alleviate mitochondrial disease. | ||
Proc Natl Acad Sci U S A A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria. | ||
J Transl Med Atrial myocyte-derived exosomal microRNA contributes to atrial fibrosis in atrial fibrillation | ||
Cell Death Dis A miR-210-3p regulon that controls the Warburg effect by modulating HIF-1α and p53 activity in triple-negative breast cancer. | ||
Mol Cell Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis
| ||
Nat Cell Biol AMPK-regulated glycerol excretion maintains metabolic crosstalk between reductive and energetic stress |
