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验证数据展示

  • WB analysis of HepG2 using 21622-1-AP

    WB analysis of HepG2 using 21622-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 21622-1-AP

    IHC staining of human small intestine using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 10x lens).

  • IHC staining of human small intestine using 21622-1-AP

    IHC staining of human small intestine using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 40x lens).

  • Featured Product
  • KD/KO验证

GPBP1 Polyclonal antibody

GPBP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21622-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

GPBP, GPBP1, SSH6, Vascular wall linked protein, VASCULIN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21622-1-AP targets GPBP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 GPBP1 fusion protein Ag14877 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GC-rich promoter binding protein 1
别名 GPBP, GPBP1, SSH6, Vascular wall linked protein, VASCULIN
计算分子量 473 aa, 53 kDa
观测分子量 45 kDa
GenBank蛋白编号BC000267
基因名称 GPBP1
Gene ID (NCBI) 65056
RRIDAB_10732822
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WP2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPBP1 antibody 21622-1-APDownload protocol
IHC protocol for GPBP1 antibody 21622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Exp Mol Med

Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.

Authors - Liang-Liang Fan
  • KD Validated
View Article