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  • Featured Product
  • KD/KO Validated

GPBP1 Polyclonal antibody

GPBP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21622-1-AP

Print datasheet

Synonyms

GPBP, GPBP1, SSH6, Vascular wall linked protein, VASCULIN

验证数据展示

  • WB analysis of HepG2 using 21622-1-AP

    WB analysis of HepG2 using 21622-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 21622-1-AP

    IHC staining of human small intestine using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

  • IHC staining of human small intestine using 21622-1-AP

    IHC staining of human small intestine using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 10x lens).

  • IHC staining of human small intestine using 21622-1-AP

    IHC staining of human small intestine using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21622-1-AP is GPBP1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen GPBP1 fusion protein Ag14877 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GC-rich promoter binding protein 1
Synonyms GPBP, GPBP1, SSH6, Vascular wall linked protein, VASCULIN
Calculated Molecular Weight 473 aa, 53 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC000267
Gene Symbol GPBP1
Gene ID (NCBI) 65056
RRIDAB_10732822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPBP1 antibody 21622-1-APDownload protocol
IHC protocol for GPBP1 antibody 21622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Exp Mol Med

Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.

Authors - Liang-Liang Fan
  • KD Validated
View Article