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验证数据展示

  • WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

    WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

  • WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

    WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

    IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

    IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

GPAA1 Polyclonal antibody, PBS Only

GPAA1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10104-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10104-1-PBS targets GPAA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0152

Product name: Recombinant human GPAA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 62-321 aa of BC004129

Sequence: EQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
别名 GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1
计算分子量 68 kDa
观测分子量 60-68 kDa
GenBank蛋白编号BC004129
基因名称 GPAA1
Gene ID (NCBI) 8733
RRIDAB_2263708
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43292
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.

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