验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
16972-1-AP targets Syntabulin in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9344 Product name: Recombinant human GOLSYN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 128-485 aa of BC012082 Sequence: SGRYMSCGENHGVRPPNPEQYLTPLQQKEVTVRHLKTKLKESERRLHERESEIVELKSQLARMREDWIEEECHRVEAQLALKEARKEIKQLKQVIETMRSSLADKDKGIQKYFVDINIQNKKLESLLQSMEMAHSGSLRDELCLDFPCDSPEKSLTLNPPLDTMADGLSLEEQVTGEGADRELLVGDSIANSTDLFDEIVTATTTESGDLELVHSTPGANVLELLPIVMGQEEGSVVVERAVQTDVVPYSPAISELIQSVLQKLQDPCPSSLASPDESEPDSMESFPESLSALVVDLTPRNPNSAILLSPVETPYANVDAEVHANRLMRELDFAACVEERLDGVIPLARGGVVRQYWS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Golgi-localized protein |
| 别名 | Golgi-localized syntaphilin-related protein, GOLSYN, KIAA1472, SYBU, Syntaxin-1-binding protein |
| 计算分子量 | 662 aa, 72 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC012082 |
| 基因名称 | GOLSYN |
| Gene ID (NCBI) | 55638 |
| RRID | AB_1939519 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NX95 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Syntabulin antibody 16972-1-AP | Download protocol |
| WB protocol for Syntabulin antibody 16972-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development. | ||
Cell Death Discov Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B | ||
Tissue Cell Targeting Siglec-15 mediates mitochondrial retrograde regulation of cervical cancer development | ||
Neurobiol Dis Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement. |
