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验证数据展示

  • WB analysis using 23153-1-AP

    WB analysis using 23153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23153-1-AP (GOLPH3L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 23153-1-AP

    IHC staining of human cervical cancer using 23153-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23153-1-AP (GOLPH3L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 23153-1-AP

    IHC staining of human cervical cancer using 23153-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23153-1-AP (GOLPH3L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GOLPH3L Polyclonal antibody

GOLPH3L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23153-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, SK-BR-3 cells, T-47D cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23153-1-AP targets GOLPH3L in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 GOLPH3L fusion protein Ag19537 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi phosphoprotein 3-like
别名
计算分子量 285 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC013870
基因名称 GOLPH3L
Gene ID (NCBI) 55204
RRIDAB_3669408
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4A5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi phosphoprotein 3 (GOLPH3) is a highly conserved membrane protein that plays an important role in maintaining the structure of the Golgi apparatus and anterograde transport. GOLPH3L(golgi phosphoprotein 3-like) is a GOLPH3 paralogue found in all vertebrate genomes. Compared with the oncogene GOLPH3, little is known about the role and function of GOLPH3L. It has been reported that GOLPH3L is highly expressed in epithelial ovarian cancer tissues, and cervical cancer tissue and is up-regulated in human rhabdomyosarcoma (PMID: 26284141; 28261346; 21822541).

实验方案

Product Specific Protocols
WB protocol for GOLPH3L antibody 23153-1-APDownload protocol
IHC protocol for GOLPH3L antibody 23153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols